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学术报告(20180611)Probing Molecular Mechanisms of Signaling Proteins with Single-Molecule Techniques
发布时间:2018-06-11

报告题目: Probing Molecular Mechanisms of Signaling Proteins with Single-Molecule Techniques
报告人: 谭砚文 教授
报告人单位: 复旦大学
报告时间: 2018年6月11日上午10:45
报告地点: 科技楼南501
报告摘要:  
  Single-molecule fluorescent spectroscopy can provide unique perspectives which complement conventional bulk biochemical approaches. We use single-molecule techniques to illuminate the transmission of signals inside the cell. Brassinosteroids (BRs) are the plant hormones that involved in numerous plant development processes. Once the signal transduction is initiated by the membrane receptor kinase, the downstream signaling pathway is realized by three proteins: BIN2 (brassinosteroid insensitive 2), BES1 (BRI1 ems suppressor1) and a kind of 14-3-3s protein. BRs signaling pathway have been extensively studied via genetics, proteomics, genomics and cell biology techniques. However, these bulk methods can’t follow the transduction process in situ or resolve molecular details at a rate matching the true signaling time-scale. Here we use a single molecular assay based on Total-Internally Reflected Fluorescence (TIRF) microscopy to observe the interaction of these three proteins. The result shows that BIN2 can phosphorylate BES1 on the order of seconds, and the dimeric 14-3-3s can only bind with BES1 in its phosphorylated form. In addition, we have, for the first time, found that the interaction between BIN2 and BES1 is oxygen dependent. This result may have implications on BRs signaling pathway’s involvement of stress acclimation in plants.
报告人简介:  
  譚硯文,复旦大学教授, 分别在国立台湾大学和哥伦比亚大学获得物理学学士和博士学位。此后,在加州大学伯克利分校和普林斯顿大学从事研究工作。2010年至今任复旦大学物理系教授、博士生导师。研究领域为实验生物物理。譚教授研究组科研方向着重在理解生物分子系统的运作原理。生物分子,尤其是蛋白质,可以在充满了变动与干扰的细胞中精确的执行多样化的功能。蛋白质分子能够高效率的运用环境中的能量,但是科学家并不理解其中的基础原理。我们将结合物理学背景与生物学中前沿的单分子萤光技术解决在生物与物理间跨领域的问题。

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